Global profiling of lncRNAs-miRNAs-mRNAs reveals differential expression of coding genes and non-coding RNAs in the lung of beagle dogs at different stages of Toxocara canis infection

The roundworm Toxocara canis causes toxocariasis in dogs and larval migrans humans. Better understanding of the lung response to T. infection could explain why must migrate undergoes part its development inside definitive host. In this study, we profiled expression patterns long non-coding RNAs (lncRNAs), microRNAs (miRNAs), mRNAs lungs Beagle infected by canis, using high throughput RNA sequencing. At 24 h p.i., 1,012 lncRNAs, 393 10 miRNAs were differentially expressed (DE). We also identified 883 DElncRNAs, 264 DEmRNAs 20 DEmiRNAs at 96 996 342 eight 36 days between control dogs. Significant changes levels transcripts related immune inflammation associated with antiparasitic canis. remarkable increase scgb1a1 all time points after suggests need for consistent moderation excessive inflammatory response. Also, upregulation foxj1 downregulation IL-1? IL-21 suggest an attenuation humoral immunity These results indicate that pathogenesis is mediated through contributions from both pro-inflammatory anti-inflammatory mechanisms. Competing endogenous (ceRNA) network analysis revealed significant interactions DEmRNAs, improved our ceRNA regulatory mechanisms context infection. data provide comprehensive networks govern reveal new mechanistic insights into interaction host parasite during course canine.